dnmt inhibitor rg108 Search Results


100 μm rg108  (FUJIFILM)
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FUJIFILM 100 μm rg108
Involvement of DNMTs in neuronal activity-dependent de novo DNA methylation. (A) Bisulfite sequencing of the hyperDMR located in the Wwc1 gene body in cells treated with DMSO or <t>RG108</t> before (DMSO) and 3 h after Bic/4AP treatment (DMSO or RG108). n = 3, * P < 0.05, Mann–Whitney U Test. (B) qRT-PCR analysis of Wwc1 treated with DMSO or RG108, 3 h after Bic/4AP treatment. n = 3, * P < 0.05, Student’s t -test. (C) Expression level of Dnmts , as analyzed using from RNA-seq data (n = 3). (D) Bisulfite sequencing of the hyperDMR located in the Wwc1 gene body in cells expressing sh Dnmt1 or/and sh Dnmt3a carried by lentivirus, 3 h after Bic/4AP treatment. n = 3, * P < 0.05, n.s.: not significant, Mann–Whitney U Test. (E) Representative images of cultured hippocampal neurons stained with antibodies against VGLUT1, PSD-95, and GFP. Scale bar: 10 µm. (F) Quantification of co-localized VGLUT1 and PSD-95 signals in (E) (n = 3, 10 neurons per replicate), n.s.: not significant, Student’s t -test.
100 μm Rg108, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dnmt+inhibitor+rg108/dnmt+inhibitor+rg108/pmc08668374-14-11-12
Average 90 stars, based on 1 article reviews
100 μm rg108 - by Bioz Stars, 2026-11
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Involvement of DNMTs in neuronal activity-dependent de novo DNA methylation. (A) Bisulfite sequencing of the hyperDMR located in the Wwc1 gene body in cells treated with DMSO or RG108 before (DMSO) and 3 h after Bic/4AP treatment (DMSO or RG108). n = 3, * P < 0.05, Mann–Whitney U Test. (B) qRT-PCR analysis of Wwc1 treated with DMSO or RG108, 3 h after Bic/4AP treatment. n = 3, * P < 0.05, Student’s t -test. (C) Expression level of Dnmts , as analyzed using from RNA-seq data (n = 3). (D) Bisulfite sequencing of the hyperDMR located in the Wwc1 gene body in cells expressing sh Dnmt1 or/and sh Dnmt3a carried by lentivirus, 3 h after Bic/4AP treatment. n = 3, * P < 0.05, n.s.: not significant, Mann–Whitney U Test. (E) Representative images of cultured hippocampal neurons stained with antibodies against VGLUT1, PSD-95, and GFP. Scale bar: 10 µm. (F) Quantification of co-localized VGLUT1 and PSD-95 signals in (E) (n = 3, 10 neurons per replicate), n.s.: not significant, Student’s t -test.

Journal: The Journal of Reproduction and Development

Article Title: Neuronal activation modulates enhancer activity of genes for excitatory synaptogenesis through de novo DNA methylation

doi: 10.1262/jrd.2021-106

Figure Lengend Snippet: Involvement of DNMTs in neuronal activity-dependent de novo DNA methylation. (A) Bisulfite sequencing of the hyperDMR located in the Wwc1 gene body in cells treated with DMSO or RG108 before (DMSO) and 3 h after Bic/4AP treatment (DMSO or RG108). n = 3, * P < 0.05, Mann–Whitney U Test. (B) qRT-PCR analysis of Wwc1 treated with DMSO or RG108, 3 h after Bic/4AP treatment. n = 3, * P < 0.05, Student’s t -test. (C) Expression level of Dnmts , as analyzed using from RNA-seq data (n = 3). (D) Bisulfite sequencing of the hyperDMR located in the Wwc1 gene body in cells expressing sh Dnmt1 or/and sh Dnmt3a carried by lentivirus, 3 h after Bic/4AP treatment. n = 3, * P < 0.05, n.s.: not significant, Mann–Whitney U Test. (E) Representative images of cultured hippocampal neurons stained with antibodies against VGLUT1, PSD-95, and GFP. Scale bar: 10 µm. (F) Quantification of co-localized VGLUT1 and PSD-95 signals in (E) (n = 3, 10 neurons per replicate), n.s.: not significant, Student’s t -test.

Article Snippet: To inhibit DNMT activity, the neurons were treated with 100 μM RG108 (Wako, Tokyo, Japan; catalog no. 041-30101) overnight.

Techniques: Activity Assay, DNA Methylation Assay, Methylation Sequencing, MANN-WHITNEY, Quantitative RT-PCR, Expressing, RNA Sequencing Assay, Cell Culture, Staining